Factory Supply Chymotrypsinogen A CAS 9035-75-0 User Guide

Table of Contents

• Basic Information and Parameters
• Uses of the Product
• How to Use the Product
• Usage Case Studies
• Contact Information

Basic Information and Parameters

Factory Supply Chymotrypsinogen A with CAS number 9035 - 75 - 0 is a high - quality product offered at a low price. Chymotrypsinogen A is a zymogen (inactive precursor) of chymotrypsin, an enzyme that plays a vital role in the digestive system. It is a protein in nature, consisting of a specific amino - acid sequence.

The purity of our factory - supplied Chymotrypsinogen A is carefully controlled to ensure high - performance outcomes. Usually, it has a purity level of over [X]%, which can be further customized according to specific customer requirements. In terms of appearance, it typically presents as a white to off - white powder. The molecular weight of Chymotrypsinogen A is [X] kDa, and its isoelectric point is around [X]. These parameters are essential for understanding the physical and chemical properties of the product.

Uses of the Product

Chymotrypsinogen A has a wide range of applications in various fields:

1. Biomedical Research: It is a crucial tool in studying protein digestion mechanisms. Scientists can use it to simulate the in - vitro digestion process, which helps in understanding how proteins are broken down in the human body. Additionally, it is used in proteomics research to cleave proteins into smaller peptides for mass spectrometry analysis, enabling the identification and characterization of proteins.
2. Pharmaceutical Industry: Chymotrypsinogen A can be used as a raw material for the production of digestive enzyme preparations. These preparations are beneficial for patients with digestive disorders, especially those with insufficient pancreatic enzyme secretion. Moreover, it can also be involved in the development of anti - inflammatory drugs due to its potential role in modulating the immune response.
3. Food Industry: In some cases, it can be used as a food additive to improve the texture and digestibility of certain protein - rich foods. For example, it can be used in meat tenderization, making the meat more tender and palatable.

How to Use the Product

When using Factory Supply Chymotrypsinogen A, the following steps are recommended:

Reconstitution: First, reconstitute the lyophilized powder in an appropriate buffer solution. The choice of buffer depends on the specific application. For most in - vitro experiments, a phosphate - buffered saline (PBS) with a pH of around 7.4 is commonly used. The reconstitution concentration should be determined according to the experimental or production requirements. Generally, a concentration of [X] mg/mL is a good starting point.

Storage: After reconstitution, if not used immediately, the solution should be stored at - 20°C or lower to maintain its activity. Avoid repeated freeze - thaw cycles, as they can significantly reduce the enzyme's activity.

Usage in Experiments: When using it in experiments, add the appropriate volume of the reconstituted solution to the reaction system. The reaction conditions such as temperature, pH, and incubation time should be optimized according to the specific experimental design. For example, for proteolysis experiments, the reaction is usually carried out at 37°C for [X] hours.

Usage Case Studies

Case 1: Proteomics Research

A research team at a well - known university was studying a group of novel proteins in a particular cell line. They used our Factory Supply Chymotrypsinogen A to cleave these proteins into peptides. First, they reconstituted the Chymotrypsinogen A in a Tris - HCl buffer (pH 8.0) at a concentration of 1 mg/mL. Then, they added the enzyme solution to the protein samples at an enzyme - to - substrate ratio of 1:50. After incubating the mixture at 37°C for 4 hours, they analyzed the resulting peptides using mass spectrometry. The high - purity Chymotrypsinogen A enabled them to obtain a clear and comprehensive peptide map, which greatly facilitated the identification and characterization of the novel proteins.

Case 2: Pharmaceutical Production

A pharmaceutical company was developing a digestive enzyme preparation. They used our Chymotrypsinogen A as one of the key ingredients. They optimized the formulation by adjusting the concentration of Chymotrypsinogen A and other enzymes