
The product we are introducing is High Quality 4-NITROPHENYL-ALPHA-D-GLUCOPYRANOSIDE with the CAS number 3767-28-0. This product is known for its high quality and comes with the best price in the market.
Chemical Formula: C12H15NO8
Molecular Weight: Approximately 301.25 g/mol
Appearance: Usually appears as a white to off - white crystalline powder
Purity: High purity, which ensures its reliability in various applications.
4 - NITROPHENYL - ALPHA - D - GLUCOPYRANOSIDE has a wide range of applications in different fields.
In the biochemical research field, it is commonly used as a substrate for the assay of alpha - glucosidase activity. Alpha - glucosidase is an important enzyme involved in carbohydrate metabolism. By using this product as a substrate, researchers can accurately measure the activity of alpha - glucosidase, which is crucial for understanding the mechanisms of carbohydrate digestion and related diseases.
In the pharmaceutical industry, it can be used in the development and quality control of drugs related to carbohydrate metabolism. For example, screening and evaluation of potential drugs that target alpha - glucosidase can be carried out with the help of this product.
When using 4 - NITROPHENYL - ALPHA - D - GLUCOPYRANOSIDE, the following steps are usually involved:
1. Preparation of the substrate solution: Weigh an appropriate amount of the product and dissolve it in a suitable buffer solution according to the experimental requirements. The concentration of the substrate solution needs to be carefully adjusted to ensure accurate results.
2. Reaction setup: Add the prepared substrate solution to the reaction system containing the enzyme or sample to be tested. Incubate the reaction mixture at the optimal temperature and pH for a certain period of time. The temperature and pH should be carefully controlled because they can significantly affect the enzyme - substrate reaction.
3. Detection: After the reaction is completed, the reaction product can be detected by appropriate methods, such as spectrophotometry. The amount of the reaction product can be correlated with the enzyme activity.
Case 1: A research team in a university was studying the effect of a new compound on alpha - glucosidase activity. They used 4 - NITROPHENYL - ALPHA - D - GLUCOPYRANOSIDE as the substrate. First, they prepared a series of substrate solutions with different concentrations. Then, they added the new compound and the enzyme to the reaction system. After incubating at 37°C for 30 minutes, they measured the absorbance of the reaction products at 405 nm using a spectrophotometer. Through this experiment, they found that the new compound could significantly inhibit the activity of alpha - glucosidase, which provided valuable information for the development of anti - diabetes drugs.
Case 2: A pharmaceutical company was conducting quality control of a new alpha - glucosidase inhibitor drug. They used 4 - NITROPHENYL - ALPHA - D - GLUCOPYRANOSIDE to measure the inhibitory effect of the drug on alpha - glucosidase. By comparing the enzyme activity in the presence and absence of the drug, they could accurately evaluate the quality and efficacy of the drug, ensuring that it met the required standards.
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This is William, CEO of Zhishang Chemical Co., Ltd.
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