Our High Quality HOLO - TRANSFERRIN, with the CAS number 11096 - 37 - 0, is a top - notch product offered at the best price in the market. Holo - transferrin is an iron - saturated form of transferrin, a glycoprotein that plays a crucial role in iron transport in the body. The purity of our product is extremely high, which ensures its reliable performance in various applications.
Physically, it is a fine powder with good solubility in appropriate buffers. It has a molecular weight that is in line with the standard characteristics of holo - transferrin. The product is stored under specific temperature conditions to maintain its stability and activity, usually recommended to be stored at - 20°C or below. This helps to preserve its biochemical properties for an extended period.
1. Cell Culture: In cell culture research, holo - transferrin is widely used as a supplement. It provides a stable source of iron for cells, which is essential for cell growth, proliferation, and metabolism. Many types of cells, such as mammalian cells and some insect cells, require iron for proper functioning. Our high - quality holo - transferrin can support healthy cell growth in culture media, leading to more reliable experimental results.
2. Biomedical Research: In the field of biomedical research, it can be used in studies related to iron metabolism. Researchers can use it to investigate how cells take up and utilize iron, which is important for understanding diseases associated with iron disorders, such as hemochromatosis and anemia.
3. Pharmaceutical Development: Pharmaceutical companies may use holo - transferrin in the development of drugs that target iron - related pathways. By using our high - purity product, they can ensure the accuracy and reproducibility of their drug development processes.
1. Preparation: Before using, thaw the product slowly at 2 - 8°C. Avoid repeated freeze - thaw cycles as this may affect the activity of the holo - transferrin. Once thawed, gently mix the solution to ensure homogeneity.
2. Dosage: The appropriate dosage depends on the specific application. For cell culture, it is recommended to start with a concentration range of 5 - 20 μg/mL in the culture medium. However, this may need to be optimized according to the cell type and experimental conditions. In biomedical research, the dosage will be determined based on the research protocol.
3. Storage: After use, if there is any remaining product, it should be aliquoted and stored at - 20°C or below again. Make sure to label the aliquots with the date and concentration for future reference.
1. Cell Culture in a Research Laboratory: A research laboratory was culturing human embryonic kidney (HEK) cells for a gene expression study. They added our holo - transferrin to the culture medium at a concentration of 10 μg/mL. The results showed that the HEK cells grew more vigorously compared to the control group without holo - transferrin. The cell viability was higher, and the gene expression levels were more stable, which was crucial for the accuracy of their study.
2. Pharmaceutical Development: A pharmaceutical company was developing a drug for iron - overload diseases. They used our holo - transferrin in in - vitro assays to study the interaction between the drug candidate and iron - binding proteins. The high purity of our product ensured that the experimental data was reliable, which accelerated the drug development process. They were able to identify the optimal dosage of the drug more quickly and move on to the next stage of clinical trials.
If you have any questions or need further information about our High Quality HOLO - TRANSFERRIN, please feel free to contact us.
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