1. Product Information

Product Name: Chapso (3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate)

CAS Number: 82473-24-3

Molecular Formula: C₃₂H₅₈N₂O₇S

Molecular Weight: 614.88 g/mol

Appearance: White to off-white powder

Purity: ≥98% (HPLC)

Storage: Store at 2-8°C in a dry, dark environment

Packaging: Available in 1g, 5g, 10g, and bulk custom packaging.

2. Applications

Chapso CAS 82473-24-3 is a zwitterionic detergent widely used in biochemical and pharmaceutical research:

• Solubilization of membrane proteins while preserving native structure and activity.
• Component of electrophoresis buffers for protein separation.
• Cell lysis and extraction of intracellular components.
• Stabilization of enzymes and receptors in diagnostic assays.

3. Usage Guide

Step-by-Step Instructions:

1. Preparation: Dissolve Chapso in distilled water or buffer (e.g., 10-50 mM concentration).
2. Concentration: Recommended working concentration: 0.1%-2% (w/v).
3. Temperature: Use at 4-25°C; avoid prolonged exposure to temperatures >37°C.
4. Compatibility: Compatible with Tris-HCl, HEPES, and phosphate buffers (pH 6-8).
5. Storage: Prepare fresh solutions or store at -20°C for long-term stability.

Safety: Wear gloves and goggles. Avoid inhalation or direct contact.

4. Case Studies

Case Study 1: Membrane Protein Extraction

A research team used Chapso (1% w/v) in 20 mM Tris-HCl buffer (pH 7.4) to solubilize G-protein coupled receptors (GPCRs) from mammalian cell membranes. The solution was centrifuged at 100,000×g for 1 hour, yielding active receptors for crystallization trials.

Case Study 2: 2D Gel Electrophoresis

Chapso was incorporated into a sample buffer (2% w/v) for resolving mitochondrial proteins. The detergent improved protein solubility without interfering with isoelectric focusing, enabling clear spot detection in Coomassie-stained gels.

5. Contact Us

For pricing, bulk orders, or technical inquiries about Chapso CAS 82473-24-3:

Email: info@vivalr.com

Phone: (86) 15866781826