
The product, High Quality 2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE with CAS number 2816-24-2, is a high - end chemical compound. The Chemical Abstracts Service (CAS) number 2816 - 24 - 2 serves as a unique identifier, which helps in accurately distinguishing this compound from other similar chemicals in scientific research and industrial applications.
It is renowned for its high quality, which means it has been produced under strict quality control measures. The purity of this product meets industry - leading standards, ensuring reliable and reproducible results in various applications. The best price associated with it makes it a cost - effective choice for both academic research institutions and industrial manufacturers.
2 - NITROPHENYL - BETA - D - GLUCOPYRANOSIDE has a wide range of uses. In the field of biochemistry, it is often used as a substrate in enzymatic assays. Enzymes such as beta - glucosidase can act on this compound, and the reaction products can be detected through colorimetric methods. This property makes it an important tool for studying enzyme kinetics, including determining enzyme activity, substrate specificity, and the effects of inhibitors or activators on enzyme function.
It also has applications in the pharmaceutical industry. It can be used in the development of new drugs, especially those targeting glycosidase - related pathways. By studying the interaction between the compound and enzymes, researchers can gain insights into potential drug targets and develop more effective therapeutic agents.
When using 2 - NITROPHENYL - BETA - D - GLUCOPYRANOSIDE, it is crucial to follow proper laboratory safety procedures. First, wear appropriate personal protective equipment, such as gloves and goggles, to prevent direct contact with the compound. The product is usually provided in a solid form. To prepare a solution, you can dissolve it in a suitable solvent. Commonly used solvents include water or buffer solutions, depending on the specific application.
For enzymatic assays, carefully measure the appropriate amount of the substrate and add it to the reaction mixture. The concentration of the substrate should be optimized according to the experimental design. Incubate the reaction mixture at the appropriate temperature and time, and then measure the absorbance of the reaction products at the specified wavelength using a spectrophotometer.
Case 1: Academic Research in Enzyme Kinetics
A research team in a university was studying the activity of a newly discovered beta - glucosidase. They used 2 - NITROPHENYL - BETA - D - GLUCOPYRANOSIDE as the substrate. By preparing different concentrations of the substrate and measuring the initial reaction rates at different enzyme concentrations, they were able to determine the Michaelis - Menten constants of the enzyme. The high - quality substrate ensured accurate and reproducible results, which were crucial for the publication of their research findings in a top - tier scientific journal.
Case 2: Pharmaceutical Development
A pharmaceutical company was screening for potential inhibitors of a glycosidase involved in a disease pathway. They used 2 - NITROPHENYL - BETA - D - GLUCOPYRANOSIDE in their high - throughput screening assays. By adding test compounds to the reaction mixture containing the substrate and the enzyme, they could quickly identify compounds that inhibited the enzyme activity. This led to the discovery of a promising lead compound for further drug development.
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