High Quality UDP-glucuronic acid CAS 2616-64-0 With Best Price
Table of Contents
1. Product Overview
UDP-glucuronic acid (CAS 2616-64-0) is a high-purity biochemical reagent widely used in enzymatic reactions and metabolic studies. Key specifications include:
- Molecular Formula: C15H22N2O18P2
- Molecular Weight: 580.29 g/mol
- Purity: ≥95% (HPLC)
- Appearance: White to off-white powder
- Storage: -20°C, dry and protected from light
- Packaging: Available in 5mg, 10mg, and custom bulk quantities
2. Applications of UDP-glucuronic acid
UDP-glucuronic acid serves as a critical substrate in:
- Enzymatic synthesis of glycosaminoglycans and proteoglycans
- Drug metabolism studies (Phase II glucuronidation reactions)
- Research on UDP-glucuronosyltransferase (UGT) enzyme activity
- Biochemical assays for plant cell wall polysaccharide biosynthesis
3. Usage Guidelines
Step-by-Step Protocol:
- Reconstitute the powder in molecular-grade water or buffer (pH 7.4) to desired concentration.
- Use in enzymatic reactions at 25-37°C with optimal UGT enzyme concentrations.
- Terminate reactions with ice-cold acetonitrile or perchloric acid.
- Store unused aliquots at -80°C for long-term stability.
Safety Note: Handle with nitrile gloves under laboratory conditions. Avoid inhalation or direct contact.
4. Detailed Use Cases
Case Study 1: In Vitro Glucuronidation Assay
Researchers added 2mM UDP-glucuronic acid to human liver microsomes containing 50μM substrate (e.g., acetaminophen). After 60-minute incubation at 37°C, LC-MS analysis showed 89% conversion to glucuronidated metabolites, validating UGT enzyme functionality.
Case Study 2: HepG2 Cell Metabolism Research
Combined with 100μg/ml alamethicin-permeabilized HepG2 cells, 1mM UDP-glucuronic acid enabled efficient membrane transport for studying intracellular glucuronidation kinetics.
5. Contact Information
For technical specifications or bulk orders:
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