The product, Factory Supply Lysozyme CAS 12650 - 88 - 3 With Low Price, is a notable offering in the market. Lysozyme is an enzyme that has a wide range of applications due to its unique properties. The CAS number 12650 - 88 - 3 serves as a unique identifier for this specific compound, allowing for clear and accurate identification in various chemical and biological databases.
In terms of its basic structure, lysozyme is a single - chain polypeptide with a well - defined three - dimensional structure. Its molecular weight typically ranges around 14 - 15 kDa. It is a globular protein with a high content of basic amino acids, which gives it certain solubility and stability characteristics. The enzyme is usually white or off - white powder in appearance, and it is soluble in water under appropriate conditions.
Lysozyme has numerous important uses in different fields. In the food industry, it is widely used as a natural preservative. It can effectively inhibit the growth of bacteria, especially Gram - positive bacteria, by hydrolyzing the peptidoglycan layer in their cell walls. This helps to extend the shelf - life of various food products such as dairy products, meat, and canned foods.
In the pharmaceutical industry, lysozyme has antibacterial, anti - inflammatory, and immune - enhancing properties. It can be used in the treatment of certain bacterial infections, as well as in the development of oral care products like toothpaste and mouthwashes to prevent oral diseases. Additionally, it has potential applications in ophthalmology, where it can be used in eye drops to prevent and treat eye infections.
In the biotechnology field, lysozyme is an essential tool for cell lysis. It can be used to break open bacterial cells to release intracellular components such as DNA, RNA, and proteins for further research and analysis.
When using lysozyme, the following steps should be followed. First, determine the appropriate concentration according to the specific application. For food preservation, the concentration usually ranges from 50 - 500 ppm. Dissolve the lysozyme powder in a suitable buffer solution at a specific pH. For most applications, a pH around 6 - 8 is appropriate. The buffer can be phosphate - buffered saline (PBS) or other commonly used buffers.
When using it for cell lysis, a higher concentration may be required. Incubate the sample with the lysozyme solution at an appropriate temperature. For many applications, an incubation temperature of 37°C is commonly used, but this may vary depending on the specific requirements. The incubation time also varies, usually ranging from 15 minutes to several hours, depending on the target cells and the purpose of the experiment.
Case 1: Food Preservation in Dairy Products
A dairy company was facing the problem of short shelf - life in its yogurt products due to bacterial contamination. By adding lysozyme at a concentration of 200 ppm to the yogurt during the production process, the company was able to significantly extend the shelf - life from 7 days to 14 days. The taste and texture of the yogurt remained unchanged, and the product quality was maintained throughout the extended storage period.
Case 2: Cell Lysis in a Biotechnology Lab
In a research laboratory, scientists were trying to extract DNA from E. coli cells. They used lysozyme to break open the cell walls. They prepared a 10 mg/mL lysozyme solution in Tris - HCl buffer (pH 7.5). After incubating the E. coli cells with the lysozyme solution at 37°C for 30 minutes, they were able to successfully lyse the cells and extract high - quality DNA for further genetic analysis.
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