Our High Quality Histamine dihydrochloride with CAS number 56 - 92 - 8 is a well - known chemical compound. Histamine dihydrochloride has a molecular formula of C5H9N3·2HCl. It appears as a white to off - white crystalline powder. The product has a high purity level, which ensures its effectiveness in various applications. The melting point of this compound is approximately 249 - 252°C (decomposition). It is freely soluble in water, making it suitable for a wide range of aqueous - based experiments and processes. Our product is offered at a low price without sacrificing quality, providing excellent value for our customers.
Histamine dihydrochloride has multiple important uses in different fields. In the medical research area, it is used to study allergic reactions and immune responses. Scientists use it to induce allergic responses in experimental models to understand the underlying mechanisms, which can contribute to the development of new drugs for treating allergies. It can also be used in the testing of anti - histamine drugs. By measuring the reaction of cells or organisms to histamine and then comparing it after the administration of an anti - histamine, researchers can evaluate the efficacy of the drug.
In the pharmaceutical industry, it serves as an intermediate in the synthesis of some drugs. For example, it can be used in the production of drugs that target the histamine receptors to regulate physiological functions such as blood vessel dilation and gastric acid secretion.
In the field of cell biology, histamine dihydrochloride is used to stimulate cells that express histamine receptors. This helps in the study of cell signaling pathways and the physiological responses of cells to external stimuli.
When using Histamine dihydrochloride, it is crucial to follow proper safety procedures. First, wear appropriate personal protective equipment, including gloves and safety goggles.
For dissolution, accurately weigh the required amount of the powder using a precise balance. Then, slowly add it to the appropriate solvent, usually water, while stirring continuously to ensure complete dissolution. It is important to control the temperature and the rate of addition to prevent any potential exothermic reactions.
When using it in experiments, carefully calculate the concentration needed according to the experimental protocol. Dilute the stock solution step - by - step if necessary. Always use calibrated pipettes or syringes for accurate dosing.
Allergy Research Use Case
In a recent allergy research project, a team of scientists used our Histamine dihydrochloride to induce an allergic reaction in a mouse model. They prepared a 1 mg/mL solution of histamine dihydrochloride in saline. Then, they injected a small amount (0.1 mL) of the solution into the skin of the mice. After injection, they observed the mice for symptoms such as redness, swelling, and increased blood flow in the injection area. By comparing these responses with those of mice treated with anti - histamine drugs, they were able to evaluate the effectiveness of the new anti - histamine compounds they were developing. The experiment was successful, and the low - price and high - quality histamine dihydrochloride played a key role in obtaining reliable results.
Cell Signaling Research Use Case
A cell biology research group was studying the histamine - induced cell signaling pathways in human mast cells. They cultured the mast cells in a suitable growth medium and then added different concentrations of histamine dihydrochloride (ranging from 0.1 μM to 10 μM) to the cell culture. By using techniques such as Western blotting and fluorescence microscopy, they were able to observe the activation of specific signaling proteins and the subsequent cellular responses. This research provided important insights into the molecular mechanisms of histamine - mediated cell activation.
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