Our product, High Quality N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic Acid with Cas 7365-44-8 and a low price, is a high - purity chemical compound. The chemical formula of this product is C₆H₁₅NO₆S. It usually appears as a white crystalline powder.
It has a molecular weight of approximately 229.25 g/mol. The melting point of this product is around 225 - 227 °C. It has good solubility in water, and the pH value of its 0.1 M aqueous solution at 25 °C is in the range of 7.0 - 8.2. This product is carefully manufactured under strict quality control standards to ensure high purity and stability, which makes it suitable for a variety of applications.
N - Tris(hydroxymethyl)methyl - 2 - aminoethanesulfonic acid, also known as TES, is widely used in the field of biochemistry and molecular biology. It is often used as a buffer in cell culture media. The buffer provides a stable pH environment for cell growth, which is crucial for maintaining cell viability and normal physiological functions.
In addition, it is used in protein purification and analysis. Many proteins are sensitive to pH changes, and TES can help maintain a constant pH during the purification process, ensuring the integrity and activity of the proteins. It is also used in enzyme - catalyzed reactions. Enzymes usually work within a specific pH range, and TES buffer can optimize the pH conditions for enzyme - substrate interactions, thereby promoting efficient enzymatic reactions.
When using N - Tris(hydroxymethyl)methyl - 2 - aminoethanesulfonic acid, first, wear appropriate protective equipment, such as gloves and goggles, to avoid direct contact with the skin and eyes. When preparing a buffer solution, accurately weigh the required amount of the product according to the desired concentration and volume of the buffer.
Dissolve the product in deionized water. Use a pH meter to monitor the pH value during the dissolution process, and adjust the pH by adding small amounts of acid or base solutions as needed. Stir the solution thoroughly to ensure complete dissolution and uniform mixing. After the buffer is prepared, it can be sterilized by filtration if necessary, especially for applications in cell culture.
Case 1: Cell Culture
In a research laboratory, scientists were culturing a particular type of mammalian cells. They used a cell culture medium containing TES buffer. By maintaining the pH of the medium at around 7.2 - 7.4, the cells grew well and showed normal morphological characteristics. Compared with the control group using a different buffer, the cells in the TES - containing medium had a higher proliferation rate and better viability, which indicated that TES buffer provided a more suitable pH environment for cell growth.
Case 2: Protein Purification
A biotech company was purifying a recombinant protein. During the ion - exchange chromatography step, they used a TES - based buffer. The buffer maintained a stable pH of 7.5, which allowed the protein to bind specifically to the chromatography column and be eluted under appropriate conditions. The purified protein had high purity and activity, which demonstrated the effectiveness of TES buffer in protein purification.
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