Product Name: 3-(4-Methoxyspiro[1,2-dioxetane-3,2'-tricyclo[3.3.1.1³,⁷]decan]-4-yl)phenyl Phosphate (AMPPD)
CAS Number: 122341-56-4
Molecular Formula: C₂₀H₂₄O₆P
Molecular Weight: 400.37 g/mol
Appearance: White to off-white crystalline powder
Purity: ≥98% (HPLC)
Storage: Store at -20°C in a dry, dark environment.
Supplier: VIVALR (GMP-certified manufacturer)
AMPPD is a chemiluminescent substrate widely used in:
- Immunoassays (e.g., Western blot, ELISA)
- Nucleic acid detection systems
- Alkaline phosphatase (ALP)-based diagnostic kits
- High-sensitivity research applications requiring low background noise
Step 1: Prepare working solution (0.1-1.0 mM in ALP reaction buffer)
Step 2: Apply to nitrocellulose membranes or microplates pre-treated with ALP-conjugated antibodies
Step 3: Incubate at 25°C for 5-30 minutes protected from light
Step 4: Detect chemiluminescence using X-ray film or CCD imaging systems
Safety: Wear PPE and operate in fume hoods. Incompatible with strong oxidizers.
Case Study 1: Western Blot Detection
Researchers at [University] utilized AMPPD to detect low-abundance proteins:
1. Transferred proteins to PVDF membrane
2. Blocked with 5% BSA for 1 hr
3. Incubated with ALP-conjugated secondary antibody (1:10,000 dilution)
4. Applied AMPPD substrate (0.5 mM in Tris-HCl buffer)
5. Captured signal within 8 minutes using chemiluminescence imager
Result: Achieved detection limit of 0.1 pg/mL target protein.
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